Live-imaging evaluation of the efficacy of elevated CO concentration in a closed cultivation system for the improvement of bioproduction in tomato fruits

Yamazaki, Haruaki*; Suzui, Nobuo; Yin, Y.-G.; Kawachi, Naoki; Ishii, Satomi; Shimada, Hiroaki*; Fujimaki, Shu

Plant Biotechnology, 32(1), p.31 - 37, 2015/04

https://doi.org/10.5511/plantbiotechnology.14.1210a

Target mutation breeding of flower color by taking advantage of ion-beam irradiation and genomic information

Tanaka, Atsushi

Plant Biotechnology, 29(3), p.191 - 192, 2012/06

https://doi.org/10.5511/plantbiotechnology.12.0002p

We launched a project to overcome the randomness of mutation and to develop logical and efficient mutation breeding by taking advantage of both ion-beam irradiation and genomic information. This research was supported by a five-year grant from the Research and Development Program for New Bio-industry Initiatives of the Bio-oriented Technology Research Advancement Institution (BRAIN). To achieve our purpose, we focused on flower-color mutations, because flavonoid biosynthesis is one of the most well-known molecular mechanisms in higher plants. We envisioned four ideal target plants for mutation breeding. These were glittering carnation, red creeping petunia, and blue-purple and crimson fragrant cyclamen. Several new factors that strongly affect flower color were identified from this project: (1) Non-acylation was found in a glittering carnation petal. (2) A loss-of-function mutation in GST (glutathione S-transferase) caused not only a decrease of pigmentation but also a change in flower color in carnation. (3) Carnation flower color was strongly affected by the function of AA5GT (acyl-glucose dependent anthocyanin 5-glucosyltransferase). (4) Delphinidins caused cyclamen flowers to turn red-purple. (5) A decrease in flavonol metabolism caused increased amounts of pigmentation in cyclamen flowers.

Isolation and characterization of suppressors of the in

Natsui, Yu*; Nefissi, R.*; Miyata, Kana*; Oda, Atsushi*; Hase, Yoshihiro; Nakagawa, Mayu*; Mizoguchi, Tsuyoshi*

Plant Biotechnology, 27(5), p.463 - 468, 2010/12

https://doi.org/10.5511/plantbiotechnology.10.0722a

EARLY FLOWERING 3 (ELF3) is a circadian clock protein with a major role in maintaining circadian rhythms in plants. In this work, was mutagenized by EMS in plants of the Lansberg background to isolate suppressors of and to understand the molecular mechanisms of flowering time controlled by ELF3. Two suppressors, obtained from this screen and from a precious study, were chosen for further analysis. Genetic mapping, gene expression analysis, and sequencing identified and as new alleles of and , respectively. Genetic interactions between and and between and in the control of the floral activator were also investigated. Six suppressor of were classified at least into four subgroups based on the expression of such floral regulators as GI, CO, FT, SVP, and FLC, and on their flowering times under LL, LD, and SD. This classification scheme is useful for the characterization of unidentified suppressor mutations.

Red-purple flower due to delphinidin 3,5-diglucosides, a novel pigment for spp., generated by ion-beam irradiation

Kondo, Emiko*; Nakayama, Masayoshi*; Kameari, Naoko*; Tanikawa, Natsu*; Morita, Yasumasa*; Akita, Yusuke; Hase, Yoshihiro; Tanaka, Atsushi; Ishizaka, Hiroshi*

Plant Biotechnology, 26(5), p.565 - 569, 2009/01

https://doi.org/10.5511/plantbiotechnology.26.565

Fragrant cyclamen cultivar ( ), that bloomed purple flower containing malvidin 3,5-diglucoside as the major anthocyanin, was irradiated with a 320-MeV carbon-ion beam at 0-16 Gy to increase flower color variation by mutation. Some of the M2 plants derived from self-pollination of M1 plants irradiated at 2 Gy were flower-color mutants that retained desirable flower shape, flower size, and leaf color. One of the mutants bloomed novel red-purple flowers, the major anthocyanin of which was delphinidin 3,5-diglucoside. Because the major anthocyanins in flowers of spp. were previously restricted to malvidin, peonidin, and cyanidin types, the generation of a cyclamen containing mostly the delphinidin-type anthocyanin is an important breakthrough in cyclamen breeding. We expect this mutant to become not only a commercial cultivar itself, but also a valuable genetic resource for cyclamen breeding.

Alteration of resistance to black Sigatoka ( Morelet) in banana by irradiation using carbon ion-beam

Reyes-Borja, W. O.*; Sotomayor, I.*; Garzn, I.*; Vera, D.*; Cedeo, M.*; Castillo, B.*; Tanaka, Atsushi; Hase, Yoshihiro; Sekozawa, Yoshihiko*; Sugaya, Sumiko*; et al.

Plant Biotechnology, 24(3), p.349 - 353, 2007/06

https://doi.org/10.5511/plantbiotechnology.24.349

Natural variation of polyglutamine repeats of a circadian clock gene in

Tajima, Takeomi*; Oda, Atsushi*; Nakagawa, Mayu; Kamada, Hiroshi*; Mizoguchi, Tsuyoshi*

Plant Biotechnology, 24(2), p.237 - 240, 2007/03

https://doi.org/10.5511/plantbiotechnology.24.237

no abstracts in English

Effects of ion beam irradiation on the regeneration and morphology of Maxim

Takahashi, Misa*; Kohama, S.*; Kondo, Komei*; Hakata, Makoto*; Hase, Yoshihiro; Shikazono, Naoya; Tanaka, Atsushi; Morikawa, Hiromichi*

Plant Biotechnology, 22(1), p.63 - 67, 2005/03

https://doi.org/10.5511/plantbiotechnology.22.63

no abstracts in English

Ion beam as a noble tool to induce apoptosis-like cell death in roots of maie (zea. mays L.)

Kawai, Maki; Kobayashi, Yasuhiko; Hirata, Aiko*; Ono, Yutaka; Watanabe, Hiroshi; Uchimiya, Hirofumi

Plant Biotechnology, 17(4), p.305 - 308, 2000/12

https://doi.org/10.5511/plantbiotechnology.17.305

no abstracts in English